Screening of antagonistic potential of Trichoderma species against seed-borne pathogenic fungi in seeds of Sorghum vulgare
Antagonistic activity of three species of Trichoderma and their isolates viz., T. viride,T. harzianum and T. hamatum against Aspergillus flavus, A. fumigatus, A. niger, A. terreus and Fusarium oxysporum of Sorghum vulgare was studied. In vitro studied were carried out in both dual culture technique and blotter test method. In dual culture technique the T. harzianum was most antagonistic ones to the seed-borne fungi followed by T. viride and T. hamatumin vitro condition. The result shows that maximum inhibition zone was created by T. harzianumagainst A. terreus, F. oxysporum, A. niger, A. Fumigatus and A. flavus. The less effective antagonistic activity in dual culture technique was recorded T. viride against all seed-borne fungi. T. harzianum antagonistic isolate as well as the commercial biocide was applied as seed treatment for controlling seed-borne mycoflora under Blotter test in vitro and Pot experiment in vivo conditions. It was observed that maximum seed germination and maximum shoot and root length recorded with A. flavus and T. harzianum combination in Pot experiment. Experiment shows, that T. harzianum antagonistic isolate was able to significant reduction in seed-borne mycoflora than T. virideand T. hamatum in Sorghum vulgare.