Morphophysiological variations in two Penicillium strains isolated from different climatic zones

  • Jyoti Department of Environmental Science, Babasaheb Bhimrao Ambedkar University, Lucknow (U.P.), INDIA
  • D. P. Singh Department of Environmental Science, Babasaheb Bhimrao Ambedkar University, Lucknow (U.P.), INDIA
Keywords: Penicillium, Climatic Zones, Morphology, Temperature, Taxonomy, Physiology

Abstract

The present investigation is a comparative study of the morphological and physiological characteristics of two Penicillium strains isolated from different climatic regions. A psychrophilic strain Penicillium oxalicum isolated from Leh (Ladakh) - a cold desert in J & K (India) was able to grow upto 4°C and other one was a  mesophilic Penicillium citrinum, isolated from Lucknow (U.P.), India, was able to grow upto 35°C. The Fungal Taxonomical classification of both the strains was primarily based on the morphology of hyphae, spores, and spore-bearing (conidial) structures of isolates. The ITS region of 18s rDNA was successfully amplified using universal primers ITS4 & ITS5 for molecular identification fungal isolates. The Psychrophilic strain was identified as Penicillium oxalicum (accession no. KR150256) and mesophilic strain as Penicillium citrinum (accession no. KR150257). Physiological studies pertaining to preference of growth temperature and nutritional (C, N) conditions on the growth of both the Penicillium strains was studied to understand their physiology response. The study revealed interesting results regarding the growth and reproductive behaviour of both Penicillium strains adapted to different climatic zones. The temperature range of 4-25°C was found to be optimum range for growth of Psychrophilic Penicillium oxalicum. However, maximum growth of the psychrophilic strain was achieved at 15°C at acidic pH 4.0. The mycelial growth of mesophilic P. citrinum occurred between 15-35°C at acidic pH 5.0; but its optimum growth was obtained between 25-30°C.  The best carbon source for the growth of P. oxalicum was glucose, followed by sucrose. On the other hand, the best carbon source for the growth of P. citrinum was found to be sucrose, followed by glucose. The best nitrogen source for growth of P. oxalicum was found to be sodium nitrate, followed by organic nitrogen glycine, and L-tryptophan. On the contrary, P. citrinum could grow well in the presence.........

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Published
2017-08-31
How to Cite
Jyoti, & Singh, D. P. (2017). Morphophysiological variations in two Penicillium strains isolated from different climatic zones. G-Journal of Environmental Science and Technology, 5(1), 13-18. Retrieved from https://gjestenv.com/index.php/gjest/article/view/80
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Articles